We are studying the modification of proteins with ADP-ribose. Quite recently, reagents have been developed to visualise this. While doing some experiments, we quickly realised that not all detection reagents are equally well suited for all methods and substrates. Therefore we decided to cross-validate the available reagents and tested them on a range of substrates; protein and RNA; in cells and in vitro. We now know which reagents to use for specific research questions, and decided to share that knowledge.
Here you find our groups’ first preprint, with a detailed characterisation of ADP-ribose detection reagents. You will also find some tips and tricks on sample generation.
The plasmids we generated will be available from Addgene, some protocols are already available on protocols.io. We’re always happy to send out materials or help out with specific questions 🙂
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