We are recruiting one or two master/medical PhD students! Below are global outlines of possible research projects, which can be further developed according to students interests.
One project has a more translational aspect and is well suited for a medical PhD student. PARP1 is utilised as drug target in for example BRCA1 negative breast cancer patients, however, the enzyme family to which PARP1 belongs, has 17 members. These enzymes can be inactivated using small molecules and may be good targets for anti-cancer therapies. We are thus planning to investigate the role of other PARP enzymes, as well as hydrolases, in diverse aspects of tumorigenesis. Methods applied will be mostly focused around cell biology, such as CRISPR/Cas-mediated knockouts, stable overexpression systems, confocal microscopy, viability, migration and proliferation assays, DNA damage and metabolic readouts.
The other project is more fundamental and well suited for a master student. We are studying both ADP-ribosyltransferases and hydrolases, which are involved in immunity. We are addressing their exact function in several model systems, including plant and human cells, and are interested in interaction with diverse pathogens including SARS-CoV-2. The student will have an opportunity to learn diverse methods, ranging from molecular cloning, biochemistry (protein purification, enzymatic assays, thin-layer chromatography) to cell biology (CRISPR/Cas, luciferase assays, confocal microscopy).